Screening for mutants with thermostabe lipase A from Burkholderia sp．ZYB002

doi:10.13343/j.cnki.wsxb.20140529

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Screening for mutants with thermostabe lipase A from Burkholderia sp．ZYB002
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                        10.13343/j.cnki.wsxb.20140529
                    
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Fund Project:Supported by the National Natural Science Funds of China (31370802)，by the Key Project from Science and Technology Bureau of Fujian Province (2013H0021) and by the Natural Science Funds for Distinguished Young Scholar of Fujian Province (2009J06013)

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Abstract:

Abstract:［Objective］We improved the thermostability of LipA from Burkholderia cecapia ZYB002 by protein engineering technology to expand the application of lipase LipA.［Method］On the basis of B-factor value of lipase LipA，series of potential mutation hotspots were selected for iterative saturation mutagenesis and the corresponding small mutation gene libraries were then constructed to screen the hyperthermal variants.［Results］ From the above mutation libraries，we obtained a series of mutants whose enzyme half-life at 55℃ increased by 1.7 to 2.2-fold．［Conclusion］B-factor iterative test (B-FIT) is feasible to mutate thermostable strains．

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Yanru Liu, Liqing Qiu, Jianzhong Huang, Bingchun Zhao, Zuozhen Wang, Xiaolan Zhu, Yuanyuan Gao, Zhengyu Shu. Screening for mutants with thermostabe lipase A from Burkholderia sp．ZYB002. [J]. Acta Microbiologica Sinica, 2015, 55(6): 748-754

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Received:November 07,2014
Revised:January 20,2015
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Online: June 03,2015
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