Interfering effect of an intergenic-derived sRNA of Cryptococcus neoformans

Abstract:

Abstract:［Objective］ We demonstrated the interfering effect of a new intergenic-derived sRNA of Cryptococcus neoformans.［Methods］We constructed a library of sRNAs from 20 to 25 nt，and used a reporter gene CLC1 that was fused to the testing sＲNAs to show the interference effect，i．e．the degradation of the CLC1 would gene rate an albino phenotype of the transformants．［Results］Through the CLC-sＲNA reporter system，we acquired one sRNA，sRNA-1，located to an intergenic region of the genome of C． neoformans，that displayed interfering effect on CLC1．［Conclusions］In C．neoformans，the origination of sＲNA may be diverse．The endogenous sＲNA should play interfering function via the canonical ＲNAi pathway．