Diversity of Bacillus species inhabiting on the surface and endophyte of lichens collected from Wuyi Mountain

Abstract:

Abstract:［Objective］The present work reported the isolation，identification and diversity of Bacillus species colonizing on the surface and endophyte in lichens collected from Wuyi Mountain．［Methods］ Nine lichen samples of Evernia，Stereocaulon，Menegazzia and other 6 genera belonging to 7 families were collected from Wuyi mountain nature reserve．The bacillus-like species colonizing on the surface and endophyte in these lichens were isolated and identified by 16S rRNA gene sequence analysis．［Results］ There was no bacillus-like species isolated from Evernia，Ramalina and Lecarona．A total of 34 bacillus-like bacteria were isolated from another 6 lichen samples． These bacteria were identified as 24 species and were classified into Bacillus，Paenibacillus， Brevibacillus， Lysinibacillus and Viridiibacillus．Paenibacillus and Bacillus are the dominant genera，and accounting for 41.2% and 35.3% of all isolated bacteria respectively． Brevibacillus，Lysinibacillus and Viridiibacillu were first reported being isolated from lichens．There were different species and quantity of bacillus colonizing on the surface and endophyte in different lichens．The quantity of bacillus colonizing on the surface of Physcia was more than 3.85×106 cfu/g and was the largest in the isolated bacteria，while the species of bacillus colonizing on the surface and endophyte in Stereocaulon was the most abundant．Most of the isolated bacteria were colonizing on (in) one lichen genera，but Paenibacillus taichungensis，Paenibacillus odorifer， Brevibacillus agri，Lysinibacillus xylanilyticus was respectively colonizing on( in) 2-3 lichen genera and Bacillus mycoides was colonizing on( in) Menegazzia，Cladonia Physcia，and Stereocaulon． ［Conclusion］There are species and quantity diversity of bacillus colonizing on(in) lichens.