Actinobacterial diversity in Xinghu Wetland

Abstract:

Abstract:［Objective］ The purpose of this study was to examine the relationship between the microbial community structure of soil actinomycetes and geochemical characteristics in Xinghu wetland sediments.［Methods］ The diversity and composition of the actiniobacterial community in sediments collected from 10 locations were studied using PCＲdenaturing gradient gel electrophoresis．Canonical correspondence analysis was carried out to examine the relationship between environmental variables and actinobacterial community composition．［Result］There were significant differences among the Shannon-wiener index，Ｒichness and Evenness．Similarity analysis of actinobacteria communities in different station showed certain trends in similarity coefficients，and actinobacteria community structure similarity of the same transect was much higher than others．According to the results of sequence analysis of DGGE dominant bands belonged to the orders Nocardioidaceae， Streptomycetaceae， Micromonosporaceae， Micrococaceae， Cellulomonadaceae and Promicromonosporaceae．Canonical correspondence analysis showed that sediment water-soluble organic carbon and available phosphorus were identified as the key factor in regulating the variations of actinobacterial community composition．［Conclusion］ Xinghu Wetland is the potential place for actinobacteria diversity．This study provides fundamental data for further studies of the underline mechanisms of structure microbial groups and the isolation of actinobacteria strains of interest．