Construction and characterization of a gspL mutant of avian pathogenic Escherichia coli

Abstract:

Abstract:［Objective］To study the role of gspL gene in avian pathogenic Escherichia coli．［Methods］The gspL mutant of Avian pathogenic Escherichia coli (APEC) was constructed by homologous recombination assay．The growth characteristics，the ability of adhesion and invasion to DF1 cells，the virulence genes transcription level and median lethal dose (LD50) were analyzed between the gspL mutant strain and the wild strain．［Results］Compared with the wild strain，the mutant strain had no significant difference in the growth status．However，its ability of adhesion and invasion was significantly lower．The transcription of genes pfs，fyuA，iss and vat increased obviously，the tsh decreased and the transcription level of luxS，ibeA，stx2f and ompA had no significant change． LD50 showed that the gspL mutant strain had 12-fold increase in virulence．［Conclusion］The deletion of gspL gene could abate the ability of adhesion and invasion，regulate and control some virulence gene transcription level，enhance the virulence of APEC． The results show that the gspL gene play roles in pathogenicity of APEC．