LAH-3，a transcriptional factor involved in osmotic regulation in Neurospora crassa

Abstract:

Abstract:［Objective］In order to identify the function of lah-3 in osmotic regulation，we generated lah-3 deletion strain and analyzed its phenotype by osmostress treatment．［Methods］We used homologous recombination to replace lah-3gene by hph gene and treated these cells with 4% NaCl and 1 M sorbitol to analyze the phenotype．Northern blot was used to detect the expressions of osmoresponsing genes．Western blot was used to examine the phosphorylation level of LAH-3 and OS-2 and the expression of OS-2．［Results］In the deletion strain of transcription factor lah-3 gene，the expressions of osmoresponsing genes gcy-1，stl-1 and pck-1 were significantly reduced． Besides，the phosphorylation level of LAH-3 protein increased under the osmostress treatment． The phosphorylation of LAH-3 was not mediated by OS-2．The deletion of lah-3 did not affect the os-2 expression and the phosphorylation level of OS-2 upon osmostress．［Conclusion］LAH-3 involved in osmoresponsing was independent of OS-2 MAPK pathway．