Molecular characteristic of duck hepatitis A virus type 1 causing pancreatitis

Abstract:

Abstract:［Objective］We studied the molecular characteristics of the full-length genome of duck hepatitis A virus type 1 causing pancreatitis in Muscovy ducklings．［Methods］We determined the entire genomic sequence of duck hepatitis A virus type 1 strain MPZJ1206 using reverse transcription polymerase chain reaction assay and analyzed the bioinformatics of the viral genome sequence.［Results］ The genome length of strain MPZJ1206 comprised 7703 bases，with a G + C content of 43.05%．The genome of MPZJ1206 contains a single，long open reading frame encoding a polypeptide of 2249 amino acids，with a genomic organization similar to those of other isolates of duck hepatitis A virus type 1．MPZJ1206 is identical with previously isolates by 93.5%－99.6% in nucleotide sequence and 97.9%－99.6% in amino acid sequence and shares genetic distance no more than 7%．Phylogenetic analysis based on genome sequence indicates that MPZJ1206 shares a close genetic relationship with two strains isolated in 2011．［Conclusion］Although pathotype caused by MPZJ1206 strain is significantly distinct from those induced by classical isolates of duck hepatitis A virus type 1，the genome of MPZJ1206 shares high homology with those of previous isolates. The change of pathotype may result from an alteration in viral tissue tropism of MPZJ1206.