Diversity of endophytic fungi associated with Ferula sinkiangensis K．M．Shen

Abstract:

Abstract:［Objective］We studied the diversity of endophytic fungi associated with Ferula sinkiangensis K．M．Shen．［Method］Endophytic fungi from different years (1-2 years，3-4 years and＞5 years) and different parts (root，stemand leaf) of Ferula sinkiangensis K．M．Shen were isolated by tissue expand method．Strains were classified by morphology and similarity of internal transcribed spacer (ITS) sequence by Clustal X method． Composition，diversity and preference of endophytic fungal community were analyzed by the isolation rate (IR)，isolation frequency ( IF)，Shannon-Wiener biodiversity index (H’)，Margalef Ｒichness index (R)．［Results］In total 140 endophytic fungi were isolated from F．sinkiangensis K．M．Shen and classified into 18 genera．Among the 140 isolates，Aureobasidium( 25.7%)，Alternaria (16.4%) and Phyllosticta (15. 7%) were the dominant genera．The isolation results show that there were some notable differences between distribution and composition of the endophytic fungi isolated from different years and different parts of Ferula sinkiangensis K．M．Shen．Meanwhile，a certain degree of years and tissue preference were also obvious.［Conclusion］ The results obtained in this study will be helpful to exploit the endophytic fungal resources of Ferula sinkiangensis K．M．Shen，which can also provide a new way for the realization of the artificial breeding of Ferula sinkiangensis K．M．Shen．