Isolation，identification of TJ430 strain and characterization of its antifungal metabolite

Abstract:

Abstract:［Obiective］To screen new agro-antibiotics，rare actinomycetes were isolated by improved separation methods from soil samples and the chemical structure of the antifungal active product was elucidated.［Methods］Dry heating method was used for soil samples pretreatment and the improved HV separation medium for rare actinomycetes separation;agar block rapid screening was used for the rapid evaluation of rare actinomycetes biological activity．For the identificationof a strain numbered TJ430，morphology observation，cell chemical composition analysis，physiological and biochemical analysis，enzymology characteristics analysis，16 S rDNA sequence analysis，and DNA hybridization method were used．Bioactive crude extract from fermentation was purified by column chromatography and preparative chromatography; infrared spectroscopy and high resolution mass spectrometry was used for structure elucidation of bioactive ingredient.［Results］A total of 570 rare actinomycetes strains were isolated．Antibacterial activity of rapid screen showed that the numbed TJ430 strain showed excellent anti oomycetes and broad-spectrum antifungal activity．Strain identification results show that the strain is a S．cavourensis．The molecular formulas of the effective ingredient is C40 H66 N3O11，molecular weight is 765．Amino，methyl，methylene，carbonyl，covalent bond，isopropyl and other chemical groups should contained in the molecular．［Conclusion］The characterized antibacterial active ingredient has good development prospect．