Effect of temperature on prodigiosin synthesis in Serratia marcecens

Abstract:

Abstract: ［Objective］ To study the effect of temperature on prodigiosin synthesis in Serratia marcecens JNB5-1．［Methods］Different proteins obtained after culturing S．marcecens JNB5-1at 28 ℃ and 37 ℃ were separated and identified through two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry．Subsequently，7 of the proteins were further analyzed by real-time qPCR．［Ｒesults］ We identified 16 different proteins． Among them，prodigiosin biosynthetic enzymes (O-methyl transferase and Oxidoreductase) and proteins related with the precursor substances (proine，methionine，serine，2-Octenal and Malonyl-CoA) of the prodigiosin biosynthetic were significantly down-expressed at 37℃．Heat shock protein (Hsp60) and superoxide dismutase were upexpressed at 37 ℃．The results of real-time qPCR show that the mRNA transcriptional levels of o-methyl transferase，oxidoreductase and transketolase at 37 ℃ were all lower than that at 28 ℃．［Conclusion］ S.marcecens JNB5-1 could produce prodigiosin at lower temperature (28℃) but not at higher temperature (37 ℃)．Higher temperature probably inhibited the expression of enzymes related with prodigiosin biosynthesis.