Biodiversity and enzymes of culturable facultativealkaliphilic actinobacteria in saline-alkaline soil in Fukang，Xinjiang

Abstract:

Abstract:［Objective］ In order to analyze the biodiversity of cultivable facultative-alkaliphilic actinobacteria and the enzymes they produced．［Methods］Total 10 soil samples were collected from saline-alkaline environments of Fukang，Xinjiang province． Facultative-alkaliphilic actinobacteria strains were isolated and identified by 16S rRNA gene sequence analysis．Enzymes including amylase，proteinase，xylanase，and cellulase were detected．［Results］ Total 116 facultative-alkaliphilic actinobacterial strains and 4 alkali-tolerant actinobacterial strains were isolated from the samples，and those strains were distributed within 22 genera in 13 families and 8 orders of actinobacteria based on their 16S rRNA gene sequence analysis．The ratio of non-predominant Streptomyces and Nocardiopsis strains were 53.3%．The positive rates of amylase，proteinase，xylanase and cellulase were 35.8，37.6，28.3 and 17.5%，respectively．［Conclusion］Diverse facultative-alkaliphilic actinobacteria were discovered from saline-alkaline environments of Fukang． Facultativealkaliphilic actinobacteria are a potential source for enzymes．The study would facilitate the knowledge of the diversity of facultative-alkaliphilic actinobacteria，and provide the technical basis for exploration of facultative-alkaliphilic actinobacteria resources.