Establishment of a genetic transformation system for Penicillium brevicompactum to produce mycophenolic acid

Abstract:

Abstract: ［Objective］ We established a genetic transformation system for Penicillium brevicompactum to produce mycophenolic acid.［Methods］We developed protoplast transformation methods mediated by Polyethylene glycol，using phleomycin resistance gene (Sh ble) as a dominant selection marker.［Result］The frequency of transformation was up to 2－3 transformants per μg DNA; analysis of the transformants by PCR showed that the foreign DNA had been integrated into the host genome．The transformants retained stable after generation.［Conclusion］The establishment of the genetic transformation system of Penicillium brevicompactum could serve as the basis for the research of molecular biology and the breeding of gene engineering of the fungus.