A hypothetical gene pair，ssl2138 and sll1092，constitutes a functional TA system on the chromosome of Synechocystis PCC 6803

Abstract:

Abstract:［Objective］We characterized the hypothetical gene pair ssl2138 and sll1092 that constitute a vapBC-family toxin-antitoxin (TA) system on the chromosome of Synechocystis PCC 6803.［Methods］An RT-PCR analysis was conducted to confirm the co-transcription of this gene pair，a selection-expression system was constructed to demonstrate the effect of their encoded proteins on E．coli growth． To validate interaction between these two proteins，an affinity capture analysis was performed．［Results］ ssl2138 and sll1092 were co-transcribed under normal growth conditions．Ectopic expression of sll1092 inhibited the growth of E．coli，which could be overcome by simultaneous or subsequent expression of ssl2138． Both proteins Ssl2138 and Sll1092 in vivo formed a protein complex due to their specific interaction．［Conclusion］ ssl2138 and sll1092 constitute a functional vapBC-family TA system of Synechocystis PCC 6803．