Progress of genome engineering technology via Clustered regularly interspaced short palindromic repeats—A review

Abstract:

Abstract:In survival competition with phage，bacteria and archaea gradually evolved the acquired immune system-Clustered regularly interspaced short palindromic repeats (CRISPR)，presenting the trait of transcribing the crRNA and the CRISPR-associated protein (Cas) to silence or cleaving the foreign double-stranded DNA specifically．In recent years，strong interest arises in prokaryotes primitive immune system and many in-depth researches are going on．Recently，researchers successfully repurposed CRISPR as an RNA-guided platform for sequence-specific gene expression，which provides a simple approach for selectively perturbing gene expression on a genome-wide scale． It will undoubtedly bring genome engineering into a more convenient and accurate new era．