Isolation，identification and characterization of five Pseudomonas strains

Abstract:

Abstract:［Objective］ We isolated Pseudomonas strains from soil samples collected from Changsha，Hunan province，catalogued them and studied the antimicrobial and antitumor activity.［Methods］We isolated Pseudomonas strains from soil samples through Galleria bait method，identified and catalogued the isolated strains according to morphological observation，physiological and biochemical characteristics and the homologous analysis of 16S rRNA sequences of nucleotides．Antimicrobial，antagonistic fungi and anti-tumor activities were studied by diffusion plate assay，dual-culture assay and cytotoxicity test respectively.［Results］We isolated 5 Pseudomonas strains from vegetable field and forest land of Changsha suburb，catalogued and named them as Pseudomonas protegens CY01，Pseudomonas chlororaphis CY02，Pseudomonas oryzihabitans CY04，Pseudomonas sp. CY05 and Pseudomonas putida CY06 respectively. P. protegens CY01 and P. chlororaphis CY02 have antibacterial activity against Bacillus subtillis and Staphylococcus aureus. P. chlororaphis CY02 has antagonistic activity against Pyricularia oryzae and anti-tumor cell activity against mouse melanoma B16 cells.［Conclusion］The isolated P. chlororaphis CY02 has a significant effect on pathogenic bacteria，Pyricularia oryzae and tumor cells.