Blue-light induced expression of S-adenosy-L-homocysteine hydrolase-like gene in Mucor amphibiorum RCS1

Abstract:

Abstract:［Objective］To determine blue-light induced expression of S-adenosyl-L-homocysteine hydrolase-like (sahhl) gene in fungus Mucor amphibiorum RCS1.［Methods］In the random process of PCR，a sequence of 555 bp was obtained from M. amphibiorum RCS1. The 555 bp sequence was labeled with digoxin to prepare the probe for northern hybridization. By northern hybridization，the transcription of sahhl gene was analyzed in M. amphibiorum RCS1 mycelia culture process from darkness to blue light to darkness. Simultaneously real-time PCR method was used to the sahhl gene expression analysis. ［Results］Compared with the sequence of sahh gene from Homo sapiens，Mus musculus and some fungi species，a high homology of the 555 bp sequence was confirmed． Therefore，the preliminary confirmation has supported that the 555 bp sequence should be sahhl gene from M． amphibiorum RCS1． Under the dark pre-culture in 24 h，a large amounts of transcript of sahhl gene in the mycelia can be detected by northern hybridization and real-time PCR in the condition of 24 h blue light． But a large amounts of transcript of sahhl gene were not found in other detection for the dark pre-culture of 48 h，even though M. amphibiorum RCS1 mycelia were induced by blue light. ［Conclusion］Blue light can induce the expression of sahhl gene in the vigorous growth of M. amphibiorum RCS1 mycelia.