Isolation of a methanol-utilizing strain and its application for determining methanol

Abstract:

Abstract:［Objective］Toisolate and characterize bacteria that can be used todevelop microbial biosensor for methanol (MeOH) determination.［Methods］ We used selective medium and streak plate to isolate bacteria． Morphological，physiological characteristics and 16S rDNA sequence analysis were used to identify the strain．AnMeOHbiosensorwas then developed by immobilizing M211 along with dissolved oxygen (O2) sensor.［Result］AnMeOH utilizing bacteriumwas isolated from biogas-producing tank using methanol as the sole carbon source，and identified as Methylobacteriumorganophilium．Decrease of O2 concentration is linearly related to the MeOH concentration in the range from 0.02% to 1% ，with the MeOH detection limit of 0.27mg /L.The response time of the biosensor is within 20 min．Furthermore，the result of interference test and the detection of methanol sample are both satisfactory．［Conclusion］Good results are obtainedin interference test and the detection of methanol sample． The proposed methodseems very attractive in monitoring methanol.