Effect of antagonistic bacteria and soil disinfectant on soil bacterium community in banana fusarium wilt disease area

Abstract:

Abstract:［Objective］The objective of the present study is to elucidate the effects of the application of cake fertilizer fermentation fluid with antagonistic bacteria and soil disinfectant chlorine dioxide on the occurrence of banana fusarium wilt disease and soil bacterium community． ［Method］Under the field cultivation conditions，the Biolog and T -RFLP method was used to investigate the soil bacterium diversity and community features in different treatments at different periods.［Result］The results show that both cake fertilizer fermentation fluid with antagonistic bacteria and soil disinfectant could reduce disease index of banana fusarium wilt disease significantly，the highest control effect could reach 60.82% with the combined application of these two methods． The result of Biolog eco plate shows that the application of cake fertilizer fermentation fluid with antagonistic bacteria could improve soil microbial AWCD(average well color development) and population uniformity，the use of soil disinfectant significantly reduced the soil microbial population’s abundance and the uniformity． Principal component analysis shows that the soil microbial population using carbon source had an increasing trend throughout the banana growing season，the main carbon sources in the early stage were amino acids，carboxylic acids，amphiphilic compounds and carbohydrates，and the increased main carbon sources in the later stage were carboxylic acids and amphiphilic compounds． Soil bacterial diversity analysis by T-RFLP shows that the treatments of cake fertilizer fermentation fluid with antagonistic bacteria had the highest bacterial TRFs (Terminal restriction fragment) fragments，which resulted from the increase of Flavobacterium，Pseudomona and Lactobacillus population in the soil.［Conclusion］The application of cake fertilizer fermentation fluid with antagonistic bacteria combining soil disinfectant could increase antagonistic microorganisms species，enhance soil microbial diversity，improve soil microbial ecological structure on the basis of reducing pathogen in soil，finally achieve the goal of improving the control effects of banana fusarium wilt disease．