Phylogenetic analysis of closely related Leuconostoc citreum species based on partial housekeeping genes

Abstract:

Abstract:［Objective］ Using the 16S rRNA，dnaA，murC and pyrG gene sequences，we identified the phylogenetic relationship among closely related Leuconostoc citreum species.［Methods］Seven Leu． citreum strains originally isolated from sourdough were characterized by PCR methods to amplify the dnaA，murC and pyrG gene sequences，which were determined to assess the suitability as phylogenetic markers．Then，we estimated the genetic distance and constructed the phylogenetic trees including 16S rRNA and above mentioned three housekeeping genes combining with published corresponding sequences.［Results］By comparing the phylogenetic trees，the topology of three housekeeping genes trees were consistent with that of 16S rRNA gene． The homology of closely related Leu． citreum species among dnaA，murC，pyrG and 16S rRNA gene sequences were different，ranged from75.5% to 97.2%，50.2% to 99.7%，65.0% to 99.8% and 98.5% 100%，respectively．［Conclusion］ The phylogenetic relationship of three housekeeping genes sequences were highly consistent with the results of 16S rRNA gene sequence，while the genetic distance of these housekeeping genes were extremely high than 16S rRNA gene． Consequently，the dnaA，murC and pyrG gene are suitable for classification and identification closely related Leu．citreum species.