Response of Nostoc flagelliforme cell to Cu2 + ，Cr2 + and Pb2 + stress

Abstract:

Abstract:［Objective］This study aimed to investigate the effects of Cu2 +，Cr2+ and Pb2 + stress on Nostoc flagelliforme cell．［Methods］ The response of Nostoc flagelliforme cell was analyzed under the stress．The modified BG11 culture medium containing different heavy metal ions of 0，0.1，1.0，10，100 mg/L was used to cultivate Nostoc flagelliforme cell at 25℃ and light intensity of 80 μmol/(m·s)．Electrolyte leakage，the activities of superoxide dismutase，the content of malondialdehyde，proline，soluble protein and trehalose were analyzed．［Results］ Under 1－100 mg/L Cu2 +，Cr2 + and Pb2 + stress，electrolyte leakage and malondialdehyde contents in Nostoc flagelliforme cell were higher than those in the control group during heavy metal ions stress． Meanwhile，superoxide dismutase activity increased slightly under 10 mg /L，but was lower afterwards．The contents of proline，soluble protein and trehalose increased under 10mg /L heavy metal ions stress，while declined under extreme heavy metal ions stress (100 mg/L)．［Conclusion］Nostoc flagelliforme cell has resistance to low heavy metal ions stress，but is damaged badly under extreme heavy metal ions stress．