Cloning and sequence characterization of replication region in plasmid pJTU112 from Micoromonospora sp．40027

Abstract:

Abstract:Micoromonospora sp．40027，the producer of fortimicin A，harbors two plasmids pJTU101 and pJTU112．［Objective］Cloning and sequencing of replication region of pJTU112，analyzing replication region sequence of pJTU112．［Methods］Different fragments of pJTU112 were cloned and introduced by conjugation into Micromonospora sp． LXH20．The replication region of pJTU112 was identified．［Results］The replication region of pJTU112 was located in the 4.7 kb SacI-KpnI DNA fragment．DNA sequencing and analysis revealed that the 4.7 kb SacI-KpnI DNA fragment encoded five open reading frames．The pJTU112.1 and pJTU112.2 were related to plasmid conjugation，pJTU112.3，pJTU112.4 and pJTU112. 5 were related to plasmid conjugation．［Conclusion］ The replication region of pJTU112 was located in the 4.7kb SacI-KpnI DNA fragment．