Use of entomopathogenic fungi to degrade wax secreted by Ceroplastes japonicus

Abstract:

Abstract:［Objective］We used entomopathogenic fungi to degrade insect wax．［Methods］We used four fungal strains，Lecanicilliurn lecanii V3. 4504，V3. 4505，Beauveria bassiana FDB01，and Metarhizium anisopliae TSL06．Wax coverings of female adults of Ceroplastes japonicus Green ( Insecta: Hemiptera: Coccoidea) were used as the sole carbon source in the mineral medium．［Results］All of the 4 strains could grow，eproduce，produce enzymes，and degrade wax．During a 7-day culture，the highest lipase activities of the 4 strains，V3.4504，V3.4505，FDB01，and TSL06 were 0.128±0.017，0.056±0.002，0.124±0.011，and 0.149±0.005 U/mL，respectively．The dehydrogenases activities of the 4 strains were 0.075±0.003，0.074±0.003，0.061±0.04，and 0.066±0.002U/mL respectively．The degradation rates of wax by the 4 strains were 18.20±0.019，11.00 ±0.011，15.4±0.017，and 23.10±0.031% ，respectively．［Conclusion］The 4 strains could depredate wax of C. japonicus．