Diversity of endophytic bacteria in rice seeds and their secretion of indole acetic acid

Abstract:

Abstract:［Objective］This study aimed to investigate the diversity of endophytic bacteria isolated from rice seeds，and screen indole acetic acid secrecting srtains．［Method］Conventional culture-dependent methods were used to isolate the endopytic bacteria from rice seeds． Phylogenetic analysis was done based on partial 16s rRNA gene sequences．The ability to indole acetic acid secretion of tested strains was analyzed qualitatively and quantitatively by colorimetry．［Result］In total 66 isolates were identified as belonging to 26 species of 15 genera of 5 phyla． Of them 26 strains were chosen to test indole acetic acid secretion． Four isolates had more ability of indole acetic acid secretion; they belonged to the genera of Staphylococcus，Rhizobium，Microbacterium and Methylobacterium．［Conclusion］The endophytic bacteria in rice seeds are diverse． Some of them could produce indole acetic acid．