Construction and characterization of ompH gene knockout mutant of avian Pasteurella multocida C48-3

Abstract:

Abstract:［Objective］To study the role of the outer membrane protein H (OmpH) in pathogenicity of avian Pasteurella multocida.［Methods］ The ompH knock-out mutant of avian P. multocida C48-3 was constructed by homologous recombination. The DNA replacement was confirmed by PCR，RT-PCR and Western blot. We compared the differences of biological characteristics such as growth rate，capsular structure，adhesion ability and virulence between the ompH knockout mutant of C48-3ΔompH and parent strain C48-3，as well as the complemented strain C48-3C.［Results］ C48- 3ΔompH was successfully constructed． Electron microscopy examination of C48-3ΔompH shows the absence of capsular material compared to the parent strain C48-3 and complemented strain C48-3C．The adhesion assay shows that the number of C48-3ΔompH adhered to CEF cells was significantly lower than that of C48-3 and C48-3C. C48-3ΔompH was relatively attenuated in mice by intraperitoneal injection.［Conclusion］The construction of C48-3ΔompH would facilitate further study on pathogenesis of avian Pasteurella multocida.