Isolation and physiological characteristics of endophytic actinobacteria from medicinal plants

Abstract:

Abstract:［Objective］To isolate，incubate and characterize cultivable endophytic antinobacteria from medicinal plants，and analyze the diversity of the endophytic antinobacteria，then explore the novel microbial resources．［Methods］Ten media were used to isolate endophytic antinobacteria from 37 fresh medicinal plant tissue samples． The optimal cultivation conditions for endophytic antinobacteria were determined by comparison． Based on the morphology of the colonies and cells of the new isolates，we chose174 isolates to analyze their 16S rRNA gene sequences and the diversity of the medicinal plant endophytic antinobacteria． The physiological characteristics of 27 representative strains were studied using Biolog GEN III MicroPlates，API 50CH and API ZYM kits．［Results］In total 940 endophytics affiliated to 47 genera of 30 families were isolated，among which more than 600 actinobacteria belonged to 34 genera and 7 unknown taxa． Good growth of the endophytic antinobacteria on PYG ( peptone-yeast-glycerol) medium with pH 7.2 at 28－32℃ was observed．Physiological characteristics differences of these isolates related to their phylogenetic relationships． Greater differences were shown among the strains from the same host plants than those from different plants grown in the same area．［Conclusion］There are great diverse endophytic actinobacteria inside the medicinal plants． No direct relationship of the endophytic actinobacteria from medicinal plants with the host plants in the sole carbon source utilization，fermentation of carbon sources to produce acid and the enzyme activities was found，while it seemed that the physiological characteristics of the isolates related to the geographical distribution of their host．