Isolate and genetic heterogeneity of psychrotrophic lactic acid bacteria from the intestinal tract of cold-water fishes from the Eerqisi river，Xinjiang

Abstract:

Abstract:［Objective］The purpose of this study was to select cold-adapted lactic acid bacteria (LAB) from the intestinal tract of cold-water fishes from the Eerqisi river Alatai． Xinjiang.［Methods］By using culture medium MRS and Elliker，isolation of lactic acid bacteria (LAB) from 9 intestinal canal of cold-water fishes was carried out. Taxonomic identity and genetic diversity of strains isolated were determined by partial 16S rRNA gene sequences and rep-PCR with three different kinds of primers named BOX，(GTG) 5，ERIC.［Results］A total of 78 ychrotrophic isolates were obtained．Among them，24 isolates had characteristics of LAB and showed the optimal growth temperature ranging from 15 to 24℃ ． The phylogenesis result showed that 24 strains belonged to 6 Genuses，Carnobacterium (3 strains)，Lactococcus (9 strains)，Enterococcus (7 strains)，Brochothrix (1 strain)，Weissella (2 strains)，Streptococcus (2 strains). rep-PCR clustering analysis showed that Lactococcus and Enterococcus strains were from different Species． Lactococcus were belonged to 4 species while Enterococcus assigned to 2 species． ［Conclusion］The phylogenetic diversity of cold-adapted LAB from the intestinal tract of cold-water fishes from the Eerqisi river in the Alatai Xinjiang was relatively abundant.