Relationship between CD4+CD25+Foxp3+regulatory T cells and Th17 responses in Chlamydia muridarum pulmonary infection

Abstract:

Abstract:［Objective］ To study the relationship between CD4+CD25+Foxp3+regulatory T cells and Th17 responses during pulmonary infection of Chlamydia muridarum (Cm) in BALB/c mice.［Methods］ BALB/c mice aged 6－8weeks were inoculated intranasally with 5×103 IFU of Cm to set up the murine model of Chlamydial pneumonia． The body weight changes，the growth of Cm and the pathology in the lung were monitored at different time post-infection． To determine the CD4+CD25+Foxp3+regulatory T cells responses in BALB/c mice，intracellular cytokine staining was used to assay the percentage of CD4+CD25+Foxp3+T cells in the spleen and mediastinum lymph node (MLN)．The production of cytokines/chemokines in the lung were monitored，including IL-6、TGF-beta、IL-17、IL-2 (by ELISA)，KC and MIP-2 (by RT-PCR).［Results］ Intranasally infected with 5×103 IFU of Cm in mice resulted in chlamydial pneumonitis featured by body weight lost，chlamydia growth and pathological damage in the lung compared with their uninfected counterparts．On day 3 post-infection，the percentage of CD4+CD25+Foxp3+T cells in the spleen and MLN were significantly decreased than the control mice; then began to increase and recover to the original level on day 7 postinfection．The production of Thl7 associated cytokines/chemokines such as IL-6，IL-17，KCand MIP-2 increased，which peaked on day 7 post-infection，then gradually reduced． The production of TGF-beta and IL-2 was consistent with the trend of CD4+CD25+Foxp3+T cells．［Conclusion］ During pulmonary infection of Cm in BALB/c mice，CD4+CD25+Foxp3+regulatory T cells may promote type 17 T cell immunity through providing TGF-beta in the presence of IL-6.