Lactobacillus plantarum NDC 75017 affects il-6 gene expression in Caco-2 cells

Abstract:

Abstract:［Objective］We tested the expression of immune-related gene interleukin 6 (il-6) in vitro to understand the influence from Lactobacillus plantarum NDC 75017 on host cells and further to reveal the regulatory mechanism．［Methods］Caco-2 cells were cocultured with L． plantarum NDC 75017 for 0，2，4，6，8，10 and 12 h，the total RNA were extracted; then the expressions of il-6 and tlr2 genes were analyzed by Real Time RT-PCR． The phosphorylation level of NF-κB was analyzed by Western Blot after the Caco-2 cells stimulation with L． plantarum NDC 75017 at 0，0. 5，1，2 and 4 h． Caco-2 cells were pretreated with pyrrolidine dithiocarbamate for 30 min before being treated with L． plantarum NDC 75017 for 2 h，then the total RNA was extracted and the expressions of il-6 and tlr2 genes were analyzed by Real Time RT-PCR． ［Results］Lactobacillus plantarum NDC 75017 could induce the expressions of il-6 and tlr2 in Caco-2 cells，the il-6 and tlr2 expressions peaked at 8 h and 6 h after cocultured with L．plantarum NDC 75017．L．plantarum NDC 75017 could rapidly activate the phosphorylation of NF-κB，and the expressions of il-6 and tlr2 were decreased notably after pretreated with pyrrolidine dithiocarbamate． ［Conclusion］L．plantarum NDC 75017 could up-regulate and then down-regulate the expression of il6 through rapidly activating tlr2-mediated NF-κB signaling pathway in Caco-2 cells．