Diversity and bioactivity of culturable actinobacteria from animal feces

Abstract:

Abstract:［Objectives］In order to provide new source for discovering new lead compounds of drugs and other products，the diversity and some bioactivities of culturable actinobacteria in animal feces were studied． ［Methods］Five animals' fecal samples were collected from Yunnan Wild Animal Park． The pure cultures of actinobacteria were isolated from these samples by using 5 different media． The 16S rRNA gene sequences of 119 selected strains were determined; the phylogenetic analysis was carried out; and antimicrobial and anti-tumor activities were determined by using agar diffusion method，tumor cell lines k562and HL60 respectively． ［Results］In total 20 genera of actinobacteria from the 5 animals' feces were identified． Many strains inhibited Bacillus subtilis，Staphylococcus lentus，Mycobacterium tuberculosis，Candida albicans and Aspergillus niger． Some strains presented antitumor activities． Some known secondary metabolites and Sannastatin，a novel macrolactam polyketide glycoside with bioactivities，were isolated and identified． ［Conclusion］Fecal actinobacteria are a new potential source for discovering drug lead and other industry products．