Screening and identification of hemicellulose degrading microorganisms in acid soil

Abstract:

Abstract:［Objective］The aim of this study was to screen hemicellulose degrading microorganisms．［Methods］ The methods used to screen the effective strains included hydrolysis spot diameter measurement of hemicellulose plate and extracellular enzyme activity． The methods used to identify the strains included culture characteristics，morphological，physiological-biochemical characteristics and molecular biological methods． ［Results］ We isolated 4 actinomycetes (NA9，NA10，NA12 and NA13)，2 fungi (NF1 and NF7) with hemicellulose degrading ability and no antagonistic effect among them． The hemicellulose degrading activity of 4 actinomyces (NA9，NA10，NA12 and NA13) was 217.6，229.8，221.1 and 211.8 U/mL． The hemicellulose degrading activity of 2 fungi (NF1 and NF7) was 217.7 and 244.2 U/mL．The hemicellulose degrading activity of complex microbial system was 299. 0 U/mL．NA9，NA10，NA12 and NA13 were Streptomyces costaricanus; NF1 was Aspergillus candidus and NF7 was Tarlaromyces flavus． ［Conclusion］the 4 actinomyces and 2 fungi screened have high hemicelluloses enzyme activity． These strains have good application value and more research value.