Stress response of Synechocystis sll0862 mutant to heat shock and oxidative stress

Abstract:

Abstract:［Objective］ To explore whether the S2P homolog，sll0862 in cyanobacterium Synechocystis sp． PCC 6803 is involved in stress response．［Methods］We compared the growth curve of sll0862 mutant and the wild type under high temperature or oxidative stress． We detected chlorophyll fluorescence under heat shock or oxidative stress by water-PAM (pulse amplitude modulated fluorometry).［Results］ Under normal condition of autotrophic growth，the growth curve of sll0862 mutant was similar with that of the wild type． However，after heat treatment at 48℃ for 30 minutes，the survival rate of sll0862 mutant was lower than that of the wild type． The sll0862 mutant hardly survived when incubated in 1 mmol/L H2O2，whereas the wild type is not affected． Meanwhile，different chlorophyll fluorescence under stress between the wild type and the mutant was observed using water-PAM．［Conclusion］ These results indicate that the S2P homology sll0862 plays an important role in response to heat shock and oxidative stress in cyanobacterium Synechocystis sp.PCC 6803，which provides foundation for further research of the sll0862 function and mechanism.