Construction of ectoine absorption defective mutant for efficient ectoine production

Abstract:

Abstract: ［Objective］To further enhance ectoine (1，4，5，6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) synthesis efficiency．［Methods］ We cloned Halomonas salina DSM 5928T teaABC gene of ectoine-specific transporter TeaABC by walking PCR and constructed ectoine absorption defective mutant H． salina DSM 5928T (teaABC－) by Red recombination technology．［Results］ The total concentration of ectoine and productivity in a 10 L fermentor were 9.10(±0.08)g/L and 9.93(±0.09)g/L.d．［Conclusion］ The ectoine absorption defect mutant H． salina DSM 5928T(teaABC－) compromised the negative feedback regulation of ectoine synthesis，which can significantly improve the efficiency of ectoine production.