Microbial community in nitrogen cycle of aquaculture water of the Pearl River Delta

Abstract:

Abstract:［Objective］In order to study the characteristic of nitrogen transport，the community structure and diversity of related microorganisms in aquaculture water of the Pearl River Delta． ［Methods］We established an artificial aquaculture v ecosystem to study the microbial community of 15N-stable isotope probing (15 N-SIP) labeled nitrogen transport microorganisms． The 15N-labeled DNA was separated by CsCl-ethidium bromide density gradient centrifugation，and was used to construct 16S rRNA gene clone libraries of bacteria and archaea． ［Results］ Phylogenetic analysis shows that 19 Operational Taxonomic Units (OTUs) from bacterial library were clustered in Proteobacteria and Planctomycetes．Proteobacteria (99.2%) was the dominant group，mainly consisted of Comamonas (15.7%) ，Nitrosomonas (12.4%)，Enterobacteriaceae (11.5%) and Nitrobacter (11.5%)．From archaeal library 9 OTUs were divided into 3 phyla:Thaumarchaeota，Crenarchaeota and Euryarchaeota． ［Conclusion］We successfully elucidated the microbial community of nitrogen transport microorganisms in aquaculture water of Pearl River Delta by using 15N-SIP． The data of the community will provide essential information for isolating nitrogen degrading microorganism，and provide scientific basis for creating a healthy aquaculture environmentl.