Sequence-based typing of enviromental Legionella pneumophila isolates in Guangzhou

Abstract:

Abstract: [Objective] To characterize the genes of Legionella pneumophila isolated from different water source in Guangzhou from 2006 to 2009. To genotype the strains by using sequence-based typing (SBT) scheme. [Methods] In total 44 L. pneumophila strains were identified by SBT with 7 diversifying genes of flaA、asd、mip、pilE、mompS、proA and neuA. Analysis of the amplicons sequence was taken in the European Working Group for Legionella Infections (EWGLI) international SBT database to obtain the allelic profiles and sequence types (STs). Serogroups were typed by latex agglutination test. [Results] Data from SBT revealed a high diversity among the strains and ST01 accounts for 30% (13/44). Fifteen new STs were discovered from 20 STs and 2 of them were newly assigned (ST887 and ST888) by EWGLI. SBT Phylogenetic tree was generated by SplitsTree and BURST programs. [Conclusion] High diversity and specificity were observed of the L. pneumophila strains in Guangzhou. SBT is useful for L. pneumophila genomic study and epidemiological surveillance.