Induced synthesis of hydroxyapatite by Aspergillus niger

Abstract:

Abstact:[Objective] The research object is to induce hydroxyapatite (HAP) synthesis using fungus. [Methods] Using the PDA (Potato Dextrose Agar Medium) liquid medium containing different concentrations of Na2HPO4 and CaCO3 to study the way Aspergillus niger synthesize HAP, and observe the induced mineral crystal structure and analysis the induced mineral type with transmission electron microscopy (TEM) and X-ray diffraction (XRD). [Resluts] The main results are as follows: (1) A. niger can induce HAP synthesis in PDA liquid medium with the proper concentration of Na2HPO4 and CaCO3. (2) The reaction of A. niger induced HAP synthesis depends on the time of the response system. Longer time is more advantageous in producing HAP. [Conclusion]The main reason for A. niger inducing HAP crystals formation are as follows: fungal metabolism produces the acidic substances to dissolve CaCO3 and the growth mycelia absorbing Ca2＋ lead to Ca2＋enriched on the surface, to promote the production of secondary mineral apatite and further transform into HAP in the mycelia spheres. Because the response condition for bio-induction HAP is temperate, the preparation craft is simple, the cost is low, the method has the potential application prospect.