Identification of Legionella isolates from environmental water by using PCR combined with enzymatic digestion analysis

Abstract:

Abstract: [Objective] To evaluate polymerase chain reaction (PCR) combined with enzymatic digestion for identification of Legionella, and distribution of Legionella in environmental water systems in Guangzhou. [Methods] Forty-four water samples collected in Guangzhou were cultivated for Legionella were identified by PCR-enzymatic digestion, 16S rDNA and mip gene sequencing analysis. [Results] Sixty-six strains of Legionella pneumophila and 46 Non-L. pneumophila were identified by PCR-enzymatic digestion and sequencing analysis. Forty-six strains of Non-L. pneumophila included 20 strains of L. feeilei, 17 L. gormanii, 7 L. oakridgensis and 2 L. longbeachae. [Conclusion] PCR combined with enzymatic digestion is a simple, rapid, and specific method for the identification of Legionella. L. pneumophila was distributed widely, followed by L. feeilei, L. gormanii, L. oakridgensis and L. longbeachae, in environmental water in Guangzhou area.