Identification and metabolism characterization of a Clostridium lituseburense strain isolated from high- altitude soil

Abstract:

Abstract: [Objective] We studied the physiological, biochemical properties and metabolism of Clostridium lituseburense P4-1 from soil in Namucuo. [Methods] We adopted Hungate anaerobic technique to get Strain P4-1 from soil in Namucuo. Through physiological, biochemical and phylogenetic analysis, we identified the strain P4-1. [Results] Cells were Gram-positive and spore-forming. It grew between 13 and 40℃ (optimum at 37℃), between pH value 5.0 and 10.0 (optimum at 7.5－8.0), and with the presence of NaCl between 0%－5%. Strain P4-1 could metabolize many carbon sources including glucose, melibiose and mannitol. Metabolites of glucose were acetate, butyrate, propionate, CO2, and little H2. Based on 16S rDNA studies, strain P4-1 was most close to Clostridium lituseburense DSM 797(M59107) with 98.7% similarity. Strain P4-1 could degrade p-toluene sulfonate. [Conclusion] Strain P4-1 tolerated low temperature, salt and could degrade p-toluene sulfonate. Its metabolites produced by fermentation of glucose could improve the soil micro-environment. It was significant for strain P4-1 to be utilized in the wastewater treatment.