Effects of S-layer proteins from lactobacillus against Salmonella typhimurium adhesion and invasion on Caco-2 cells

Abstract:

Abstract: [Objective] S-layer proteins of Lactobacillus acidophilus were extracted and purified, then the effect of Lactobacillus acidophilus and its S-layer proteins against the adhesion and invasion of salmonella typhimurium to caco-2 cells were investigated. [Methods] S-layer proteins were purified by anion-exchange column {diethylaminoethyl (DEAE) DE52}, and the inhibition of Lactobacillus acidophilus and its S-layer proteins were studied against the adhesion and invasion of salmonella typhimurium on Caco-2 cells. [Results] S-layer proteins exhibited strongly inhibitory effects of adhesive and invading properties of Salmonella typhimurium. In the adhesive experiments (competitive, exclusive and displacement), Salmonella typhimurium adhesion was reduced by S-layer proteins and the ability of adherence to Caco-2 cells were 1.17 %±5.97 %, 8.71 %±1.36 % and 10.56 %±0.92 % , respectively (p＜0.01). The influence to inhibit the competitive adhesion of Salmonella typhimurium was optimal. Furthermore, the S-layer proteins showed a stronger effect than Lactobacillus acidophilus to inhibit Salmonella typhimurium adhesion on Caco-2 monolayers (p＜0.01). Moreover, invasion of Salmonella typhimurium to Caco-2 monolayers was inhibited by S-layer proteins. [Conclusion] S-layer proteins inhibited adherence and invasion of Salmonella typhimurium. The result can merit a highlight for preventive or probiotic therapy in human or animals with disease caused by Salmonella typhimurium.