Cloning and expression of prenyl transferase gene of Metarhizium ansopliae

Abstract:

Abstract: [Objective ] To clone and identify prenyl transferase gene from Metarhizium anisopliae and to understand the gene structure and expression. [Methods ] Using Switching Mechanism At 5' end of RNA Transcript (SMART) method, we isolated the full length cDNA sequence and DNA sequence. ?Then we used quantitative RT-PCR analysis of the gene expression levels at different stages of colonization of host hemolymph by M. anisopliae. [ Results] The Mpt gene had two exons and one intron and the CDS was 1026 bp which encoded a protein with 341 amino acid residues; qRT-PCR analysis showed that the gene expression levels were significantly different, especially highly up-regulated at the late stages. [ Conclusion] The Mpt gene was successfully cloned from M. anisopliae for the first time and the gene had the characteristic of high expression levels at the late stages.