Bacterial diversity in a hot gas spring soil of MaNasi County, Xinjiang by culture-independent approach

Abstract:

Abstract：[Objective] In order to investigate bacteria diversity in a hot gas spring soil of MaNasi county, Xinjiang. [Methods] Total DNA were directly extracted from the soil of a hot gas spring from Xinjiang.16S rDNA were amplified directly from purified DNA by PCR with universally bacteria-specific rDNA primers and cloned. Positive clones were identified by restriction fragment length polymorphism(RFLP), and unique rDNA types clones were sequenced, analysed and then constructed phylogenetic tree. [Results] Twenty-nine operational taxonomic units (OTU) from 170 positive clones were found belonging to 6 phyla: Firmicutes, Proteobacteria, Acidobacteria, Bacteroidetes, Planctomycetes, Actinobacteria. Firmicutes (71%) was the absolutely dominant components of the soil bacterial community. 14 sequences (about 1.5 kb) from 29 OTUs showed less affiliation with known taxa(<97% sequence similarity).[Conclusion] Soil bacterial diversity is low in the hot gas spring soil, but exit a large number of new unknown taxon in this environment.