Construction of cDNA library of protozoan ciliates Euplotes octocarinatus

Abstract:

Abstract: The protein biosynthesis in cells is an open process cooperatively regulated by many protein factors and enzymes, which form a complicated protein network and signal transduction pathway for mRNA metabolism and protein translation. [Objective] To provide a platform for studying on the function of proteins involving in process of translation termination and the relationship among these proteins in ciliates. [Methods] We constructed the cDNA library of protozoan ciliates Euplotes octocarinatus strictly following the procedure of BD MatchmakerTM Library Construction & Screening kit from Clontech. [Results] We obtained a cDNA library of ciliates Euplotes, the titer of which was -2.437×107cfu/mL, which could suffice for functional gene screen. By using the class II polypeptide release factor (eRF3) as bait protein, we obtained some putative genes, including a partial cDNA putatively encoding RNA helicase. [Conclusion] This library will provide a convenient platform for identifying the functional genes in ciliates.