Comparison of bacterial and archaeal community of mangrove soil under different vegetation in Dongzhaigang，Hainan Island

Abstract:

Abstract:［Objective］ We compared bacterial and archaeal diversity and community structure of mangrove soil under different vegetation，and to reveal better understanding of microbial resources． ［Methods］ Bacterial and archaeal 16S rRNA gene libraries were constructed and analyzed for soils under Kandelia candel trees，Sonneratia apetala trees，and naked tideland，in Dongzhaigang Mangrove National Nature Reserve of Hainan Island． Template DNA was directly extracted from soil samples． PCR were amplified using primers 27F /1492R (bacterial) and Arch21F/Arch958R (archaeal)．［Results］A total of 16 phyla dominated by Proteobacteria and Chloroflexi were detected in bacterial libraries，and 6 groups of Crenarchaeota and 7 groups of Euryarchaeota，predominated by Marine Benthic Group C and Marine Benthic Group D，respectively were found in archaeal libraries． Shannon-Wiener index (H') and Schao1 estimator indicated that soil microbial diversity under the introduced species Sonneratia apetala was much lower than indigenous species Kandelia candel，even lower than naked tidal flat sediment near mangrove． Distinct differences in microbial community structure under different vegetation were observed． Soil microbial community structure under Kandelia candel was much similar with that of naked tideland.［Conclusion］ Mangrove soil contained rich population of bacteria and archaea; there existed distinct differences in mangrove soil microbial community structure and diversity among different vegetation．