Development and application of a new p1-based genotyping method for Mycoplasma pneumoniae

Abstract:

Abstract:［Objective］ To develop a PCR method for detecting the newly reported genotype (variant 2c，V2c) of Mycoplasma pneumoniae strains． ［Methods］Specific primer was designed for detecting the V2c type based on the variant region of V2c strain p1 gene． A nested multiple PCR method for V2c strain detection was set up and confirmed by related gene sequencing． In total 214 clinical strains isolated from Beijing between 2008 and 2011 were analyzed by this typing method． ［Results］Nest multiple PCR typing method is effective to detect the V2c strain． Of the 214 M． pneumoniae strains 90. 2% (193/214) were type 1，0.9% (2/214) were variant 2a，and 8. 9%(19/214) were V2c． No type 2 was detected． ［Conclusion］This typing method is effective to distinguish the V2c strains from other variant M． pneumoniae strains，and important for the epidemiological study of Mycoplasma pneumonia infection．