Comparing the primer specificity for betaproteobacterial ammonia-oxidizing bacteria in recirculation aquaculture systems

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Comparing the primer specificity for betaproteobacterial ammonia-oxidizing bacteria in recirculation aquaculture systems
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Fund Project:Supported by the National Programs for High Technology Research and Development of China (2007AA10Z409)，by the Zhejiang Provincial Natural Science Foundation of China (Y3090046) and by the Academic Discipline Project of Ningbo University (xkl09120)

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Abstract:［Objective］ To analyze the difference of specificity of four primers for betaproteobacterial ammonia-oxidizing bacteria (β-AOB) 16S rDNA gene from the biofilm of closed recirculation aquaculture systems．［Methods］We used 16S rDNA clone libraries to describe the β-AOB diversity．［Results］ CTO189f /CTO654r produced the highest frequency of β-AOB-like sequences (67.3%)．The amplification performance of primer was noticeably influenced by the biofilm samples． Hereinto，the biofilm of closed recirculation aquaculture systems of Tilapia nilotica resulted in the higher amplification performance of primers．［Conclusion］ CTO189f /CTO654r exhibited the highest specific for β-AOB 16S rDNA gene from the biofilm of closed recirculation aquaculture systems．

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Fangfang Pei, Peng Zhu, Hang Min, Kaihong Lu, Yangfang Ye. Comparing the primer specificity for betaproteobacterial ammonia-oxidizing bacteria in recirculation aquaculture systems. [J]. Acta Microbiologica Sinica, 2011, 51(10): 1342-1350

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Received:April 05,2011
Revised:June 03,2011
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