Distribution of microbes and their hereditary diversity in the rhizosphere of carnation in greenhouse

Abstract:

Abstract：[Objective] To investigate the relationship between microbial community and the blight diseases in rhizosphere of carnation for biological control. [Methods] Bacterial strains isolated from the rhizosphere of healthy and blight carnation plants in greenhouse were replicated by morphology and 16S rRNA gene similarity, and investigated by using phylogenetic analysis based on 16S rRNA gene sequence comparisons. [Results] Isolates belonged to 4 phyla of bacteria, 65 isolates from the samples of healthy carnation plants belonged to 9 genera and Bacillus, Streptomyces, Mortierella as the dominant bacteria. However, 33 isolates from the samples of blight carnation plants belonged to 12 genera and Stenotrophomonas, Sphingobacterium, Pseudomonas, Chryseobacterium, Amycolatopsis and Fusarium only from the sample of blight carnation plants. At least 13 isolates should represent potential novel species based on lower similarites of 16 S rRNA gene (90-98%). [Conclusion] The result showed that either the percentage of the fungus in the total strains or the abundance of Bacillus groups in the total strains can be the referential targets to evaluate whether the carnation soil would be healthy for the carnational growth or lead to fusarium wilt diseases, accurately forecasting potential risks of the disease.