Diversity of culturable actinomycetes in Sea deposit of Tiger beach at Bohai bay, Dalian, China

Abstract:

Abstract: [Objective] To study the diversity of culturable actinomycetes isolated from the sea deposit of Tiger beach at Bohai bay, Dalian, China. [Methods] By using five different media strains we isolated actinomycetes strains from the sea deposit of Tiger beach at Bohai bay, Dalian, China. Partial 16S rRNA sequences were carried out to characterize the diversity of culturable actinomycetes. [Results] A total of 1215 colonies with phenotypical actinomycetes were isolated, of which 271 were classified by 16S rRNA phylogenetic analysis. The data showed that 251 strains were homologous with Actinobacteria (92.26%) including 15 genera of 11 families, and the remaining 20 strains were possible members of the phylum Firmicutes and Proteobacteria. There were 7 strains (3-166, 3-117, 3-435, 5-186-2, 5-9, 5-171, 3-134) to be preliminarily identified as unreported species. [Conclusion] A high diversity of culturable actinomycets both in terms of the number of species and phylogenetic composition was presented from the sea deposit of Tiger beach in Bohai bay.