Construction and identification of virB2 deletion mutants of Brucella vaccine strain M5-90

Abstract:

Abstract: [Objective] To construct Brucella vaccine strain M5-90ΔvirB2 deletion mutants with attenuated virulence. [Methods] We constructed the homologous recombination plasmid pGEM-7zf-ΔvirB2-sacB by using traditional molecular biology technology and then obtained M5-90ΔvirB2 strains by ampicillin and sucrose screens after electroporation. The deletion mutants were identified by PCR. Its stability were detected by continuous bacteria culture. [Results] The virB2 deletion mutant strains were successfully constructed. The reverse mutation did not occur within 10 passages. [Conclusion] The results of this study will based on the development of gene deletion attenuated vaccine of Brucella.