Comparison of Six Molecular Methods for Differentiation of Lactococcus lactis subsp. lactis and cremoris

Abstract:

Abstract: [Objective] To compare six molecular methods for differentiation of Lactococcus lactis subsp. lactis and cremoris. [Methods] Six molecular methods, such as 16S rRNA gene analysis, 16S-23S rRNA Intergenic spacer region polymorphism, Denaturing Gradient Gel Electrophoresis (DGGE), Random Amplified Polymorphic DNA (RAPD), Repetitive Extragenic Palindromic-PCR and Restricted Fragment Length Polymorphisms were used to differentiate the reference strains. [Results] Except for 16S rRNA gene analysis and 16S-23S rRNA Intergenic spacer region polymorphism, the other protocols were competent. [Conclusion] The methods of DGGE and RAPD were more appropriate for differentiation of Lactococcus lactis subsp. lactis and cremoris.