Cloning and analysis of the telomeres of five Streptomyces linear plasmids

Abstract:

Abstract: Streptomyces strains were isolated from soil samples of Tibet, five small linear plasmids were detected by pulsed-field gel electrophoresis. [Objective] Cloning, sequencing and analysis of telomeres of these plasmids. [Methods] The telomeres were cloned by a modified procedure — “alkaline treatment and enzyme digestion in gels”. [Results] Telomeres of five linear plasmids were cloned and sequenced. Compared with the typical Streptomyces telomeres, the newly identified telomeres contained multiple palindromes, but some could not “fold-back” of their first conserved palindrome I with the internal palindromes to form a “super-hairpin”, and palindromes of some telomeres did not contain the 3-nt “loop”. [Conclusion] New telomere sequences were cloned by a modified procedure. Both folding-back of the telomere palindromes and 3-nt loop of palindromes varied among telomeres.