Development of whole-genome DNA microarray of Vibrio parahaemolyticus and evaluation of the microarray quality

Abstract:

Abstract：[Objective] Our aim in this study was to develop a whole-genome DNA microarray of Vibrio parahaemolyticus, build up a method of microarray hybridization and carry on microarray quality evaluation. [Methods] Based on the genomic sequences of V. parahaemolyticus, we chose a total number of 4770 genes, amplified them by PCR with specific primers, purified the PCR products and printed them onto glass slides. We performed two sets of hybridizations by the method of two-fluorescence comparative hybridization to evaluate the microarray quality, followed by PCR method to validate parts of microarray results. [Results] Microarray hybridization results were completely consistent with theory expectations and PCR verification results. [Conclusion] We successfully developed a batch of good quality whole-genome DNA microarrays of V. parahaemolyticus, built up a method of microarray-based comparative genomic hybridization of V. parahaemolyticus and a set of microarray data analysis standard method.